Journal:

Article Title: Restricted V gene usage and VH/VL pairing of mouse humoral response against the N-terminal immunodominant epitope of the amyloid ? peptide

doi: 10.1016/j.molimm.2010.09.012

Figure Lengend Snippet: Kinetic constants and affinities of various immobilized rFab with Aβ 1–16 -Im7.

Article Snippet: Two different assay formats were designed to analyze the binding of Fab to Aβ: (1) Capture Assay : recombinant Aβ 1–16 -Im7 fusion protein, used as a “surrogate Aβ antigen” (for details, see Section 3), was injected over Fab captured on the surface of a GLC chip containing previously immobilized Human Fab Binder (GE Healthcare); and (2) Direct Assay : recombinant Fabs were injected over various Aβ forms captured on a NeutrAvidin coated chip (NLC, BioRad).

Techniques: Mutagenesis

Journal:

Article Title: Restricted V gene usage and VH/VL pairing of mouse humoral response against the N-terminal immunodominant epitope of the amyloid ? peptide

doi: 10.1016/j.molimm.2010.09.012

Figure Lengend Snippet: Kinetic constants and affinities of various rFab with immobilized biotin-Aβ 1–16 (bAβ 1–16 ) and biotinylated Aβ 1–42 HMW oligomers (bAβ 1–42 -HMW oligomers).

Article Snippet: Two different assay formats were designed to analyze the binding of Fab to Aβ: (1) Capture Assay : recombinant Aβ 1–16 -Im7 fusion protein, used as a “surrogate Aβ antigen” (for details, see Section 3), was injected over Fab captured on the surface of a GLC chip containing previously immobilized Human Fab Binder (GE Healthcare); and (2) Direct Assay : recombinant Fabs were injected over various Aβ forms captured on a NeutrAvidin coated chip (NLC, BioRad).

Techniques: